Journal: Tissue Engineering and Regenerative Medicine
Article Title: Hypoxic Stress Induces Complement-Mediated Lysis of Mesenchymal Stem Cells by Downregulating Factor H and CD59
doi: 10.1007/s13770-024-00678-6
Figure Lengend Snippet: Exposure to severe hypoxia enhanced complement system activation within the MSCs. The expression level of the complement component markers C3b, iC3b and C5-C9 were analyzed using Elisa assay. By comparing the expression levels of the complement components C3b, iC3b, and C5-C9 in MSCs under hypoxic and normoxic conditions, the ELISA assay can identify dysregulation of complement activation in response to severe hypoxia. A C3b expression levels were significantly increased in hypoxic MSCs compared to control. B iC3b expression levels decreased significantly in hypoxic MSCs compared to normoxic cells. D C5-C9 expression levels were significantly higher in hypoxic MSCs compared to control. (*p < 0.05 vs. control, n = 3)
Article Snippet: ELISA assays were performed to detect the expression and activity of C3b (Genochem world SL., lot # GW3605Hu), iC3b (My BioSource, Cat # MBS1603937), and MAC complex (C5b-C9) (GenoChem, Cat # GW3858Hu), following the manufacturer's instructions.
Techniques: Activation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Control